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 E-Mail IMAGE: Conventional epifluorescence (left) and super-resolved localisation microscopy images of gut bacteria (Escherichia coli), using the new RhoBAST-dye marker complex for fluorescence labelling. Scale bar: 1 μm. view more  Credit: Heidelberg University/Karlsruhe Institute of Technology Ribonucleic acid (RNA) is key to various fundamental biological processes. It transfers genetic information, translates it into proteins or supports gene regulation. To achieve a more detailed understanding of the precise functions it performs, researchers based at Heidelberg University and at the Karlsruhe Institute of Technology (KIT) have devised a new fluorescence imaging method which enables live-cell RNA imaging with unprecedented resolution. The method is based on a novel molecular marker called Rhodamine-Binding Aptamer for Super-Resolution Imaging Techniques (RhoBAST). This RNA-based fluorescence marker is used in combination with the dye rhodamine. Due to their dist

Baden wubergGerd ulrich nienhausAndres jaeschkeMurat sunbulGerman research foundationInstitute of applied physicsInstitute of pharmacyHelmholtz associationKarlsruhe institute of technologyHeidelberg universityKarlsruhe instituteRhodamine binding aptamerSuper resolution imaging techniquesMolecular biotechnologyApplied physicsChemistry physics materials sciences