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Operator
Good afternoon, ladies and gentlemen, and welcome to the C.H. Robinson First Quarter 2021 Conference Call. [Operator Instructions] As a reminder, this conference is being recorded Tuesday, April 27, 2021.
I would now like to turn the conference over to Chuck Ives, Director of Investor Relations. Please go ahead.
Chuck Ives
Director of Investor Relations
Thank you, Donna, and good afternoon, everyone. On the call with me today is Bob Biesterfeld, our Chief Executive Officer; and Mike Zechmeister, our Chief Financial Officer. Bob and Mike will provide a summary of our 2021 first quarter results, and we will then open the call up for questions. This change to live Q&A from our previous practice of responding to pre-submitted questions is in response to valued input from our analysts and shareholders. Our earnings presentation slides are supplemental to our earnings release and today s comments and can be found in the Investor Relations section of our website at chrobins
Strong truckload business drives profit growth for C.H. Robinson
C.H. Robinson Worldwide, Inc. today reported financial results for the quarter ended March 31, 2021.
First Quarter Key Metrics:
Gross profits increased 23.7 percent to $697.7 million.
Adjusted gross profits(1) increased 23.7 percent to $702.4 million.
Income from operations increased 104.1 percent to $223.3 million.
Adjusted operating margin
Diluted earnings per share (EPS) increased 124.6 percent to $1.28.
Cash flow from operations decreased $115.2 million to $56.7 million used by operations.
(1) Adjusted gross profits and adjusted operating margin are Non-GAAP financial measures. The same factors described in this release that impacted these Non-GAAP measures also impacted the comparable GAAP measures. Refer to page 9 for further discussion and a GAAP to Non-GAAP reconciliation.
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Operator
Good morning, ladies and gentlemen, and welcome to the C.H. Robinson Fourth Quarter 2020 Conference Call. [Operator Instructions] Following today s presentation, Chuck Ives will facilitate a review of previously submitted questions. [Operator Instructions] As a reminder, this conference is being recorded Wednesday, January 27, 2021.
I would now like to turn the conference over to Chuck Ives, Director of Investor Relations.
Chuck Ives
Director, Investor Relations
Thank you, Donna, and good morning everyone. On the call with me today is Bob Biesterfeld, our Chief Executive Officer; and Mike Zechmeister, our Chief Financial Officer. Bob and Mike will provide a summary of our 2020 fourth quarter results, and we will follow their comments with responses to the pre-submitted questions that we received after our earnings release yesterday.