Scientists develop new method for fluorescence microscopy :

Scientists develop new method for fluorescence microscopy
ANI |
Updated: Jan 03, 2021 11:31 IST
Tokushima [Japan], January 3 (ANI): Scientists from the Institute of Post-Led Photonics, Tokushima University develop a fluorescence lifetime microscopy technique that uses frequency combs and no mechanical parts to observe dynamic biological phenomena.
Fluorescence microscopy is widely used in biochemistry and life sciences because it allows scientists to directly observe cells and certain compounds in and around them. Fluorescent molecules absorb light within a specific wavelength range and then re-emit it at the longer wavelength range.
However, the major limitation of conventional fluorescence microscopytechniques is that the results are very difficult to evaluate quantitatively; fluorescence intensity is significantly affected by both experimental conditions and the concentration of the fluorescent substance.