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Cumulative expression of heterologous XlnR regulatory modules and AraRA731V in Penicillium oxalicum enhances saccharification efficiency of corn stover and corn fiber | Biotechnology for Biofuels and Bioproducts

Penicillium oxalicum engineered strain DB2 and its mutant strains with multiple regulatory modules were constructed. Mutant strain RE-4-2 with two regulatory modules showed a significant increase in the reducing sugar released from corn stover and corn fiber as well as in the conversion of cellulose than DB2. RE-5-2 with three regulatory modules showed a further increase in reducing sugar released from corn stover and the conversion of cellulose on the basis of RE-4-2. RE-4-2-AraRA731V constructed by overexpressing AraRA731V in RE-4-2 showed an increase of 7.2 times and 1.2 times in arabinofuranosidase and xylosidase activities, respectively. Reducing sugar yield and cellulose conversion of corn stover and corn fiber by RE-4-2-AraRA731V were further increased.

China , United-states , Shanghai , Coomassie , Ashanti , Ghana , Endocellulase-cmcase , Microbiological-culture-collection-center , Sangon-biotech , Roche-real-time ,

Discovery of novel alkaline-tolerant xylanases from fecal microbiota of dairy cows | Biotechnology for Biofuels and Bioproducts

Xylo-oligosaccharides (XOS) are considered as a promising type of prebiotics that can be used in foods, feeds, and healthcare products. Xylanases play a key role in the production of XOS from xylan. In this study, we conducted a metagenomic analysis of the fecal microbiota from dairy cows fed with different types of fodders. Despite the diversity in their diets, the main phyla observed in all fecal microbiota were Firmicutes and Bacteroidetes. At the genus level, one group of dairy cows that were fed probiotic fermented herbal mixture-containing fodders displayed decreased abundance of Methanobrevibacter and increased growth of beneficial Akkermansia bacteria. Additionally, this group exhibited a high microbial richness and diversity. Through our analysis, we obtained a comprehensive dataset comprising over 280,000 carbohydrate-active enzyme genes. Among these, we identified a total of 163 potential xylanase genes and subsequently expressed 34 of them in Escherichia coli. Out of the 34 expressed genes, two alkaline xylanases with excellent temperature stability and pH tolerance were obtained. Notably, CDW-xyl-8 exhibited xylanase activity of 96.1 ± 7.5 U/mg protein, with an optimal working temperature of 55 ℃ and optimal pH of 8.0. CDW-xyl-16 displayed an activity of 427.3 ± 9.1 U/mg protein with an optimal pH of 8.5 and an optimal temperature at 40 ℃. Bioinformatic analyses and structural modeling suggest that CDW-xyl-8 belongs to GH10 family xylanase, and CDW-xyl-16 is a GH11 family xylanase. Both enzymes have the ability to hydrolyze beechwood xylan and produce XOS. In conclusion, this metagenomic study provides valuable insights into the fecal microbiota composition of dairy cows fed different fodder types, revealing main microbial groups and demonstrating the abundance of xylanases. Furthermore, the characterization of two novel xylanases highlights their potential application in XOS production.

Xiaomei , Zhejiang , China , United-states , Taigong , Guizhou , Anhui , Nanjing , Jiangsu , Hubei , Guangdong , Switzerland

Comprehensive insights into the mechanism of keratin degradation and exploitation of keratinase to enhance the bioaccessibility of soybean protein | Biotechnology for Biofuels and Bioproducts

Keratin is a recalcitrant protein and can be decomposed in nature. However, the mechanism of keratin degradation is still not well understood. In this study, Bacillus sp. 8A6 can completely degrade the feather in 20 h, which is an efficient keratin degrader reported so far. Comprehensive transcriptome analysis continuously tracks the metabolism of Bacillus sp. 8A6 throughout its growth in feather medium. It reveals for the first time how the strain can acquire nutrients and energy in an oligotrophic feather medium for proliferation in the early stage. Then, the degradation of the outer lipid layer of feather can expose the internal keratin structure for disulfide bonds reduction by sulfite from the newly identified sulfite metabolic pathway, disulfide reductases and iron uptake. The resulting weakened keratin has been further proposedly de-assembled by the S9 protease and hydrolyzed by synergistic effects of the endo, exo and oligo-proteases from S1, S8, M3, M14, M20, M24, M42, M84 and T3 families. Finally, bioaccessible peptides and amino acids are generated and transported for strain growth. The keratinase has been applied for soybean hydrolysis, which generates 2234 peptides and 559.93 mg/L17 amino acids. Therefore, the keratinases, inducing from the poultry waste, have great potential to be further applied for producing bioaccessible peptides and amino acids for feed industry.

Qingdao , Shandong , China , Kyoto , Japan , Suzhou , Jiangsu , Sangon-biotech-shanghai-co , Standard-testing-group-co , Bacillus-genetic-stock-centre , Ohio-state-university

Six-hour time-restricted feeding inhibits lung cancer progression and reshapes circadian metabolism | BMC Medicine

Accumulating evidence has suggested an oncogenic effect of diurnal disruption on cancer progression. To test whether targeting circadian rhythm by dietary strategy suppressed lung cancer progression, we adopted 6-h time-restricted feeding (TRF) paradigm to elucidate whether and how TRF impacts lung cancer progression. This study used multiple lung cancer cell lines, two xenograft mouse models, and a chemical-treated mouse lung cancer model. Stable TIM-knockdown and TIM-overexpressing A549 cells were constructed. Cancer behaviors in vitro were determined by colony formation, EdU proliferation, wound healing, transwell migration, flow cytometer, and CCK8 assays. Immunofluorescence, pathology examinations, and targeted metabolomics were also used in tumor cells and tissues. mCherry-GFP-LC3 plasmid was used to detect autophagic flux. We found for the first time that compared to normal ad libitum feeding, 6-h TRF inhibited lung cancer progression and reprogrammed the rhythms of metabolites or genes involved in glycolysis and the circadian rhythm in tumors. After TRF intervention, only timeless (TIM) gene among five lung cancer-associated clock genes was found to consistently align rhythm of tumor cells to that of tumor tissues. Further, we demonstrated that the anti-tumor effect upon TRF was partially mediated by the rhythmic downregulation of the TIM and the subsequent activation of autophagy. Combining TRF with TIM inhibition further enhanced the anti-tumor effect, comparable to treatment efficacy of chemotherapy in xenograft model. Six-hour TRF inhibits lung cancer progression and reshapes circadian metabolism, which is partially mediated by the rhythmic downregulation of the TIM and the subsequent upregulation of autophagy.

Chicago , Illinois , United-states , Wuhan , Hubei , China , Lipo , Hainan , Tokyo , Japan , Wuxi , Jiangsu

Novo Holdings participates in $290m strategic financing of Sangon Biotech

Novo Holdings participates in $290m strategic financing of Sangon Biotech
tmcnet.com - get the latest breaking news, showbiz & celebrity photos, sport news & rumours, viral videos and top stories from tmcnet.com Daily Mail and Mail on Sunday newspapers.

Huagai , Sichuan , China , Denmark , Copenhagen , Køavn- , Novo-nordisk , Tracey-wang , Amit-kakar , China-merchant-health , Novo-nordisk-foundation , Novo-holdings

Frontiers | Diversity of common bean rhizobia in blackland of northeastern China and their symbiotic compatibility with two host varieties

Common bean (Phaseolus vulgaris L.) is an important crop in the world that forms root nodules with diverse rhizobia. Aiming to learning the rhizobial communities associated with common bean in the black soil of Northeast China, 79 rhizobia were isolated from root nodules of two host varieties (Cuican and Jiadouwang) grown in two sites of blackland, and were characterized by comparative sequence analyses of 16S rRNA, recA, atpD, nodC, nifH genes and whole genome. As results, Rhizobium indigoferae, R. anhuiense and R. croatiense as minor groups and three dominant novel Rhizobium species were identified based on their ANI and DDH values to the type strains of relative species. This community composition of rhizobia associated with common bean in the tested black soils was unique. Despite their different species affiliation, all of them were identified into the symbiovar phaseoli according to the phylogenies of symbiotic genes (nodC and nifH). While the discrepancies found in nodC and nifH genotypes evidenced that the evolutions of nodulation (nod) and nitrogen fixation (nif) genes were partially independent. In addition, only one dominant rhizobial species was shared by the two common bean varieties grown in the two soil samples, implying that both the plant variety and the soil characters affected the compatibility between rhizobia and their hosts. These findings further enlarged the spectrum of common bean-nodulating rhizobia and added more information about the interactions among the soil factors, rhizobial species, and host plants in the symbiosis.

Brazil , Ethiopia , Beijing , China , Malawi , Binxian , Heilongjiang , Spain , Mexico , Muling , Zhejiang , Inner-mongolia-autonomous-region

Efficient production of icariin and baohuoside I from Epimedium Folium flavonoids by fungal α-l-rhamnosidase hydrolysing regioselectively the terminal rhamnose of epimedin C | Biotechnology for Biofuels and Bioproducts

Industrial application of icariin and baohuoside I has been hindered by the short supply to a great extent. In this work, a novel GH78 α-l-rhamnosidase AmRha catalyzed the bioconversion of low-value epimedin C in crude Epimedium Folium flavonoids (EFs) to icariin and baohuoside I was developed. Firstly, the high-level expression of AmRha in Komagataella phaffii GS115 attained an enzyme activity of 571.04 U/mL. The purified recombinant AmRha could hydrolyze α-1,2-rhamnoside bond between two rhamnoses (α-Rha(2 → 1)α-Rha) in epimedin C to produce icariin with a molar conversion rate of 92.3%, in vitro. Furtherly, the biotransformation of epimedin C to icariin by the recombinant Komagataella phaffii GS115 cells was also investigated, which elevated the EFs concentration by fivefold. In addition, biotransformation of epimedins A-C and icariin in the raw EFs to baohuoside I was fulfilled by a collaboration of AmRha and β-glucosidase/β-xylosidase Dth3. The results obtained here provide a new insight into the preparation of high-value products icariin and baohuoside I from cheap raw EFs.

China , Chengdu , Sichuan , Switzerland , Germany , Nanjing , Jiangsu , Shaanxi , Shanghai , United-states , Lianyungang , Swiss

GL Capital Group leads $290m investment in Chinese life sciences firm Sangon Biotech

CITIC Private Equity, Greenwoods Investment, and Huagai Capital invested.

Huagai , Sichuan , China , Hong-kong , Shanghai , Qiming , Chinese , David-qisong-wang , Jeffrey-li , Life-sciences , China-development-bank-capital , Citic

Angiopep-2 modified exosomes load rifampicin

Loading Rifampin into exosomes and modified the exosomes with a brain-targeting peptide to improve blood-brain barrier permeability of Rifampin

China , San-diego , California , United-states , Wuxi , Jiangsu , Hangzhou , Zhejiang , Nanoview-biosciences , Exoview-nanoview-biosciences , Ltd-wuxi , China-co-ltd

Targeted therapy for triple-negative breast cancer

Dual target of EGFR and mTOR suppresses triple-negative breast cancer cell growth by regulating the phosphorylation of mTOR downstream proteins. Read more...

Xinjiang , Jiangxi , China , United-states , Franklin-lake , New-jersey , New-york , United-kingdom , Texas , Beijing , California , Abcam-cambridge