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Automated nucleic acid sample QC in NGS workflows

High-quality DNA and RNA samples support strong next-generation sequencing (NGS) results. To help prevent suboptimal sequencing data and avoid wasting time and resources, quality control (QC) steps are recommended at various points in the NGS workflow, from initial sample assessment to final library qualification for NGS library preparation protocols. Here, Agilent Technologies explains how the benefits of the Agilent TapeStation systems and ScreenTape technology offer ideal and easy-to-use s
Agilent Tapestation Agilent Technologies Application Note Nucleic Acid Quality Control Agilent Technologies

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Simultaneous LC/MS analysis of ultra-short through long chain PFAS compounds (C1-C10) using multi-modal chromatography

Perfluoroalkyl and polyfluoroalkyl compounds (PFAS) are a structurally diverse class of manmade chemicals utilized in a variety of manufacturing processes since the 1960s. Due to their hydrophobic and lipophobic moieties, these compounds have high mobility in water and soil allowing their proliferation throughout the environment; further, the high bond strength of the C-F bond renders many PFAS indestructible in common environmental conditions and in human bodies, leading to bioaccumulation. Due
Application Note Hilicpak Vt 50 2d Showa Denko

Analysis of organic acids and oxyhalides (SI-35 4D)

In this application note from Showa Denko, Shodex IC SI-35 4D (a column for rapid oxyhalides analysis) was used for the analysis of seven common anions and some organic acids. The long elution time (i.e., more than 30 minutes for succinic acid and malonic acid) required by IC SI-52 4E was shortened to 15 minutes using IC SI-35 4D. Therefore, IC SI-35 4D is also suitable for the rapid analysis of organic acids.
Showa Denko Application Note Ion Chromatography Showa Denko Hodex Ic Si 35 4d Organic Acids

Single-cell dispensing and screening of cell lines for monoclonality verification using the impedance-based single-cell dispenser and high-throughput fluorescence-based imager

There is an unmet need in the industry for a device that allows the fast and efficient isolation of single cells while preserving their integrity and providing insurance for their clonality. Performing gene editing, single-cell dispensing, and screening to develop a stable monoclonal cell line is a long and labor-intensive process. The optimization of these steps through different approaches, such as high-throughput screening and/or automation, can increase the efficiency and yield of the monocl
Application Note Molecular Devices Single Cell Dispensing Onoclonality Verification High Throughput Molecular Devices

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