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(U)HPLC analysis of biomolecules

This compendium, from Tosoh Bioscience, compiles more than twenty application notes covering important aspects of biopharmaceutical analysis such as protein aggregation, charge isoforms and glycosylation as well as determination of drug-to-antibody ratio of antibody-drug-conjugates. The last section of the notebook provides tips and tricks to improve chromatographic separation of biomolecules.
Tosoh Bioscience Application Note

Evaluation of different ion-pairing reagents

The effect of various ion pairing reagents (amines and acidic counterions) on the LC-UV and LC-MS analysis of oligonucleotides (ONs) has been studied. The type of amine and concentration of the counterion play a significant role in the retention and resolution of the ONs and strongly affect the MS data.  In this application note, Agilent Technologies demonstrates that there are numerous variables available for optimization of the analytical conditions for ONs. The analyses were
Agilent Technologies Application Note Hplc Ms

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