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Real-time monitoring of microbial dynamics during fermentation is essential for wine quality control. This study developed a method that combines the fluorescent dye propidium monoazide (PMA) with CELL-qPCR, which can distinguish between dead and live microbes for Lactiplantibacillus plantarum. This method could detect the quantity of microbes efficiently and rapidly without DNA extraction during wine fermentation. The results showed that (1) the PMA-CELL-qPCR enumeration method developed for L....

Climate change, pest infestation, and soil degradation are significantly reducing wheat (Triticum aestivum L.) yield. Wheat is cultivated in rice-wheat and cotton-wheat cropping systems and escalating global population is exerting substantial pressure on the efficiency of these systems. Conservation tillage and crop rotation could help in lowering soil degradation and pest infestation, and improving wheat yield. This three-year study evaluated soil properties, weed infestation and wheat yield un...

Common bean (Phaseolus vulgaris L.) is an important crop in the world that forms root nodules with diverse rhizobia. Aiming to learning the rhizobial communities associated with common bean in the black soil of Northeast China, 79 rhizobia were isolated from root nodules of two host varieties (Cuican and Jiadouwang) grown in two sites of blackland, and were characterized by comparative sequence analyses of 16S rRNA, recA, atpD, nodC, nifH genes and whole genome. As results, Rhizobium indigoferae...

Aflatoxins (AFT) identified as a Group 1 human carcinogen naturally contaminate various types of food and could increase the risk of hepatocellular carcinoma (HCC) through dietary intake. Chongqing municipality is located in Southwest China with subtropical monsoon climate which is conducive to AFT contamination in crops. However, the burden of HCC caused by the dietary exposure of the population in Chongqing to AFT has not been quantified. We estimated the burden of HCC in terms of Disability A...

Agri-food value chains that generate an increased willingness to pay among consumers by offering enhanced levels of sustainability are widely discussed as an important strategy for creating new business opportunities and fostering food system transformation. Previous research has highlighted the importance of governance arrangements to secure the trust necessary for the establishment of sustainability-based agri-food value chains. However, how different coordination designs by private and public...

Practical and theoretical advancements have not caught pace with rising scientific researches in the rapidly emerging economy undertaking a shift to a more sustainable economy, particularly green economy. After the UN adopted the 2030 Agenda for Sustainable Development, there has been a surge in interest in the green economy among academics around the world, and the literature on the issue is proliferating. This paper adopts the methodology of systematic review and thematic analysis to summarize...

A combination of factors including migration, climate change and the encroachment of growing cities are associated with the extinction of species and a reduction in biodiversity. In Mexico maize landraces are an important source of germplasm that could be invaluable for future breeding programs in response a changing environment. Efforts to develop conservation strategies are hampered by the costs of housing and maintaining large germplasm collections. Effective criteria are therefore needed to ...

In Ethiopia, pesticides are widely used in vegetable production. However, if used incorrectly they may harm consumers of vegetables contaminated with pesticide residues, as well as producers handling the pesticides and lead to ecological damage. We performed a cross-sectional survey to assess pesticide residues level in vegetables produced in central and eastern Ethiopia. A total of 232 vegetable samples (91 tomatoes, 106 cabbages and 35 Swiss chard) were collected from fields and retail markets...

In the Southwestern Atlantic, the Falkland Current intrudes onto the South American shelf, resulting in the meeting of two water masses which are completely different in temperature and dynamic characteristics, thus generating the Southwestern Atlantic Front (SAF). Therefore, the SAF has prominent characteristics of thermal and dynamics. The current ocean front detection is mainly by performing gradient operations on sea surface temperature (SST) data, where regions with large temperature gradie...

Vaccines are a critical tool for the control strategy for foot-and-mouth disease (FMD) in Mongolia where sporadic outbreaks regularly occur. A two-dose primary vaccination course is recommended for most commercial vaccines though this can be logistically challenging to deliver among nomadic pastoralist systems which predominate in the country. Although there is evidence that very high potency vaccines can provide prolonged duration of immunity, this has not been demonstrated under field conditio...