Here, the heat treatment for FeCoCrNiAl high-entropy alloy (HEA) laser cladding (LC) layer was given at a temperature which is higher than that of the first phase transition. The effects of heat treatment on the phase transition, microstructure and mechanical properties of the LC-ed FeCoCrNiAl were studied. Meanwhile, the LC-ed sample was compared with the casting specimen. The results showed that the cladding layer had lower activation energy of phase transformation, and it had higher proportion of Fe element because of the dilution of the matrix metal, which promoted the formation of face-centered cubic (FCC) phase. Then more FCC phase structures precipitated in the cladding layer after heat treatment. However, the formation of FCC phase did not change the texture characteristics of the cladding layer. The differences in physical properties, slip system, Burgers vector and dislocation types between the two phases, as well as the serious atomic disarrangement at the two-phase interfac