Plant breeding field trials are typically arranged as a row by column rectangular lattice. They have been widely analysed using linear mixed models in which low order autoregressive integrated moving average (ARIMA) time series models, and the subclass of separable lattice processes, are used to account for two-dimensional spatial dependence between the plot errors. A separable first order autoregressive model has been shown to be particularly useful in the analysis of plant breeding trials. Recently, tensor product penalised splines (TPS) have been proposed to model two-dimensional smooth variation in field trial data. This represents a non-stochastic smoothing approach which is in contrast to the autoregressive (AR) approach which models a stochastic covariance structure between the lattice of errors. This paper compares the AR and TPS methods empirically for a large set of early generation plant breeding trials. Here, the fitted models include information on genetic relatedness amon