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Frontiers | A rapid and reliable method for the determination of Lactiplantibacillus plantarum during wine fermentation based on PMA-CELL-qPCR

Real-time monitoring of microbial dynamics during fermentation is essential for wine quality control. This study developed a method that combines the fluorescent dye propidium monoazide (PMA) with CELL-qPCR, which can distinguish between dead and live microbes for Lactiplantibacillus plantarum. This method could detect the quantity of microbes efficiently and rapidly without DNA extraction during wine fermentation. The results showed that (1) the PMA-CELL-qPCR enumeration method developed for L....
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Evolving from a marginal territory to a radiation centre

I RECENTLY came across an article in National Geographic on June 13, 2023, introducing the โ€˜Burma Roadโ€™ in Yunnan (not to be confused with the one in Penang). During World War II, a remarkable feat was achieved by a legion of 200,000 Chinese labourers who constructed this famous 717-mile truck ro
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