Domesticated chickens (Gallus gallus domesticus) are a dominant part of the global human diet. Although the early domestication history of this species remains disputed, Mainland Southeast Asia (MSEA) is assumed to have been the initial domestication center. The eastward spread of chickens into Island Southeast Asia (ISEA) and the Pacific is typically attributed to human-mediated dispersals. Chicken remains are relatively common at Pacific Neolithic sites but are extremely rare in the archaeological records of MSEA and ISEA. Therefore, the exact routes and timing of the human-mediated spread of chickens from their native range in MSEA into the Pacific remain questions of interest. Here, we present the earliest evidence of Gallus on the Indonesian island of Flores at Liang Bua. This site has yielded an extensive stratigraphic sequence that spans from ~190,000 calendar years (ka) ago until the present and includes dense accumulations of faunal remains. Twelve bones from the cave's H