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IMAGE: Conventional epifluorescence (left) and super-resolved localisation microscopy images of gut bacteria (Escherichia coli), using the new RhoBAST-dye marker complex for fluorescence labelling. Scale bar: 1 μm. view more
Credit: Heidelberg University/Karlsruhe Institute of Technology
Ribonucleic acid (RNA) is key to various fundamental biological processes. It transfers genetic information, translates it into proteins or supports gene regulation. To achieve a more detailed understanding of the precise functions it performs, researchers based at Heidelberg University and at the Karlsruhe Institute of Technology (KIT) have devised a new fluorescence imaging method which enables live-cell RNA imaging with unprecedented resolution.
The method is based on a novel molecular marker called Rhodamine-Binding Aptamer for Super-Resolution Imaging Techniques (RhoBAST). This RNA-based fluorescence marker is used in combination with the dye rhodamine. Due to their dist
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Organic molecules that capture photons and convert these into electricity have important applications for producing green energy. Light-harvesting complexes need two semiconductors, an electron donor and an acceptor. How well they work is measured by their quantum efficiency, the rate by which photons are converted into electron-hole pairs.
Quantum efficiency is lower than optimal if there is self-quenching , where one molecule excited by an incoming photon donates some of its energy to an identical non-excited molecule, yielding two molecules at an intermediate energy state too low to produce an electron-hole pair. But if electron donors and acceptors are better spaced out, self-quenching is limited, so that quantum efficiency improves.
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Neil Palmer / International Center for Tropical Agriculture
Nature Food warns that heat stress in animals caused by rising temperatures and humidity will occur more frequently and for longer periods, impacting milk and meat productivity for dairy cattle, beef cattle, sheep, goat, pigs, and poultry across East Africa.
Analysis of historical data from 1981-2010 shows that livestock have already been progressively subjected to heat stress conditions. According to projections, this is set to worsen significantly, with severe implications for livestock unable to cope with the extra heat.
Authors warn that four to 19 percent of current meat and milk production occurs in areas where dangerous heat stress conditions are likely to increase in frequency from 2071-2100. If climate conditions persist at their current accelerated rate, elevated levels of heat stress could make much of East Africa unsuitable for exotic pig, poultry, and dairy cattle production unless direct and immediate acti