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Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, China Ebola virus infection causes severe hemorrhagic fever, and its mortality rates varied from 25 to 90% in the previous outbreaks. The highly infectious and lethal nature of this virus highlights the need for reliable and sensitive diagnostic methods to distinguish it from other diseases present with similar clinical symptoms. Based on multiplex polymerase chain reaction (PCR) and oligonucleotide microarray technology, a cost-effective, multipathogen and high-throughput method was developed for simultaneous detection of Ebola virus and other pathogens associated with hemorrhagic fever, including Marburg virus, Lassa fever virus, Junin virus, Machupo virus, Rift Valley fever virus, Crimean-Congo hemorrhagic fever virus, malaria parasite, hantavirus, severe fever with thrombocytopenia syndrome virus, dengue virus, yellow fever virus, Chikungunya virus, influenza A virus, and influenza B virus. This assay had a

South koreaUnited statesDemocratic republic of the congoSierra leoneEl halasChemiluminescent imager academy of military medical sciencesSangon biotech co ltdZhejiang provincial centerTechnology program of zhejiang provinceDepartment of microbiologyHealth leading talents program of zhejiang provinceSubstrate millipore corporationAldrich coWorld health organizationDevelopment of chinaNational key research