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Purpose: The deposition of hydroxyapatite (HAp) crystals plays an important role in the development of vascular calcification (VC). This study aimed to demonstrate the effects of nanosized HAp (nHAp) on vascular smooth muscle cells (VSMCs) and VC progression. Methods: Transmission electron microscopy (TEM) was used to examine cellular uptake of nHAp. Cell viability was determined using CCK-8 assay kit. Mitochondrial impairment and reactive oxygen species were detected by TEM and fluorescence dy...

CircRNA-TBC1D4, circRNA-NAALAD2 and circRNA-TGFBR3: selected key circRNAs in neuroblastoma and their associations with clinical features

15 Basal epithelial cells are a blood–prostate barrier between luminal cells and basement membrane 16 but are absent in prostate carcinoma. 17–19 Loss of basal epithelial cells is a characteristic of prostate carcinoma 20,21 and one of the diagnostic criteria of prostate cancer, 19 suggesting that some essential connections might exist between basal epithelial cells and prostate cancer cells. So, we put out a question regarding whether basal epithelial cells could suppress migration or inva...

Knocking Down KCNQ1OT1 Suppressed LPS-Induced Neuroinflammation and Neuronal Apoptosis in HMC3 Cells For further exploring the function of KCNQ1OT1, we transfected HMC3 cells with pc-KCNQ1OT1 or si-KCNQ1OT1. The expression of KCNQ1OT1 was evaluated by RT-qPCR. Compared with control cells (cells transfected with pc-NC or si-NC), the expression of KCNQ1OT1 in HMC3 cells transfected with pc-KCNQ1OT1 was significantly increased, while the expression of KCNQ1OT1 in cells transfected with si-KCNQ1OT1...

9 Aberrantly expressed CCDC144NL-AS1 was observed in gastric cancer, 9 however, its roles in HCC development and the potential mechanism have not been reported yet. In this study, we investigated the expression pattern of CCDC144NL-AS1 using human HCC tissues, and explored the underlying mechanism from the epigenetic pathway. We also estimated the therapy value of CCDC144NL-AS1 using HCC mouse model. Our study may provide novel therapeutic targets for HCC treatment to improve the prognosis of H...

1* 1Department of Medicine, Division of Pulmonary, Allergy, and Critical Care Medicine, University of Alabama at Birmingham, Birmingham, AL, United States 2Department of Dermatology, University of Alabama at Birmingham, Birmingham, AL, United States 3Department of Biomedical Engineering, University of Alabama at Birmingham, Birmingham, AL, United States 4Department of Pathology, Division of Molecular and Cellular Pathology, University of Alabama at Birmingham, Birmingham, AL, United States Tumo...

−1), which is consistent with the results in HepG2 cells (0.2–2 mg·mL −1), 55 but promoted cell proliferation at low concentrations in vitro. Notably, the cell viability was greater than 60% when the CS-CD concentration was less than 4.5 µg•mL −1, which may be desired (regeneration, neurogenesis, etc.) or undesired (tumours and cancers) cell proliferation. Because the type of cell proliferation is unknown, it is difficult to determine whether toxicity is high or low. Further study o...

Discussion There are several therapeutic approaches to bio-functional foods against cardiovascular diseases, both in experimental and clinical studies. Germinated Brown Rice (GBR) exerted its protective properties on cardiovascular diseases and related-cardiovascular metabolism dysfunction, including hypertension, hyperlipidemia, and diabetes. Here, this is the first study to represent the cardioprotective effect of GBR on cardiomyocyte-simulated I/R injury. The significant findings in this stu...

Figure 1 Construction of occludin/ZO1 probes and analysis of their efficacy and motility. ( A) In cpstFRET (cpVenus-7aa-cpCerulean), cpCerulean (cyan) is the donor and cpVenus (yellow) is the acceptor. The probes have high Förster resonance energy transfer (FRET) efficiency when donor and acceptor are parallel without external force (f: external force). The external force reduces the FRET efficiency by changing the angle of cpstFRET. ( B) Schematic representation of the occludin/ZO1 probe cons...

2Laboratory for Pharmaceutical Biotechnology, University of Ghent, Ghent, Belgium 3ProGenTomics, Ghent, Belgium 4Laboratory of Proteinscience, Proteomics and Epigenetic Signaling, Faculty of Pharmaceutical, Biomedical and Veterinary Sciences, University of Antwerp, Antwerp, Belgium 5Department of Biomedical Sciences, University of Antwerp, Antwerp, Belgium Chikungunya virus (CHIKV) is an arbovirus with a global spread and significant public health impact. It is a positive stranded RNA alphaviru...