Drought poses a major challenge to potato production worldwide. Climate change is predicted to further aggravate this challenge by intensifying potato crop exposure to increased drought severity and frequency. There is an ongoing effort to adapt our potato production systems through development of drought-tolerant cultivars that are appropriately engineered for the changing environment. Breeding of drought- tolerant cultivars can be approached through the identification of drought-related physiological and biochemical traits and their deployment in new potato cultivars. Thus, the main objective of this study was to develop a method to identify and characterize potato genotypes tolerant to drought and related key traits. To achieve this objective, first we studied 56 potato genotypes including 51 cultivars and 5 advanced breeding lines to assess drought tolerance in terms of tuber yield in the greenhouse experiment. Drought differentially reduced tuber yield in all genotypes. Based on t