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Precise correction of Duchenne muscular dystrophy exon deletion mutations by base and prime editing

Abstract Duchenne muscular dystrophy (DMD) is a fatal muscle disease caused by the lack of dystrophin, which maintains muscle membrane integrity. We used an adenine base editor (ABE) to modify splice donor sites of the dystrophin gene, causing skipping of a common DMD deletion mutation of exon 51 (∆Ex51) in cardiomyocytes derived from human induced pluripotent stem cells, restoring dystrophin expression. Prime editing was also capable of reframing the dystrophin open reading frame in these ca...
United States Matrigel Corning E Welker Addgene F Zhang Addgene Millipore Amicon Versene Gibco

Population genomic evidence of Plasmodium vivax Southeast Asian origin

P. vivax originates from sub-Saharan Africa based on the circulation of its closest genetic relatives ( P. vivax-like) among African great apes. However, the limited number of genetic markers and samples investigated questions the robustness of this hypothesis. Here, we extensively characterized the genomic variations of 447 human P. vivax strains and 19 ape P. vivax-like strains collected worldwide. Phylogenetic relationships between human and ape Plasmodium strains revealed that P. vivax-...
Coted Ivoire Oromia Regional State Ivory Coast Centre National De National Center College Of Health

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