Results of the study for positive cerebrospinal fluid cultures, provides valuable strategies for preventing pathogens and improving evidence-based treatment.
Purpose: The deposition of hydroxyapatite (HAp) crystals plays an important role in the development of vascular calcification (VC). This study aimed to demonstrate the effects of nanosized HAp (nHAp) on vascular smooth muscle cells (VSMCs) and VC progression.
Methods: Transmission electron microscopy (TEM) was used to examine cellular uptake of nHAp. Cell viability was determined using CCK-8 assay kit. Mitochondrial impairment and reactive oxygen species were detected by TEM and fluorescence dye staining, respectively. Cell apoptosis was detected by Western blot analysis and Annexin V staining. Mouse model of VC was built via applying nHAp on the surface of abdominal aorta. Calcification was visualized by Alizarin red and von Kossa staining.
Chinese scientists discover gene associated with epilepsy
1 2021-05-10 16:18:32Xinhua
Editor : Li Yan
ECNS App Download Screenshot from the journal Brain s website shows the study, led by the Second Affiliated Hospital of Guangzhou Medical University, on finding novel UNC13B variants associated with partial epilepsy. (Xinhua)
Chinese scientists have recently discovered a gene responsible for epilepsy, a neurological disorder of the brain.
The new study published in the international journal Brain described the gene as UNC13B, which has the loss-of-function variants that can cause intractable seizures and abnormal sensations and behaviors.
Using gene screening methods, a research team led by the Second Affiliated Hospital of Guangzhou Medical University in south China s Guangdong Province, found novel UNC13B variants in eight unrelated families from 446 cases with partial epilepsy.
2021-05-08 11:35:54 GMT2021-05-08 19:35:54(Beijing Time) Xinhua English
GUANGZHOU, May 8 (Xinhua) Chinese scientists have recently discovered a gene responsible for epilepsy, a neurological disorder of the brain.
The new study published in the international journal Brain described the gene as UNC13B, which has the loss-of-function variants that can cause intractable seizures and abnormal sensations and behaviors.
Using gene screening methods, a research team led by the Second Affiliated Hospital of Guangzhou Medical University in south China s Guangdong Province, found novel UNC13B variants in eight unrelated families from 446 cases with partial epilepsy.
According to researchers, in some patients, partial epilepsy has variable causes such as trauma, infection, immune abnormalities or neoplasm. However, in most cases the cause was previously unknown.
Immunohistochemistry
Renal cancer and normal tissue were used for immunohistochemistry. After baking for 60 min in a constant temperature oven at 60°C, tissue chips were dewaxed in xylene and hydrated in graded alcohols. Slides were blocked for 5 min, then primary antibody Gαi1 (1:100, Proteintech), Ki67 (1:8000, Proteintech) was added for incubating at 4°C overnight. After washing, secondary antibody HRP Goat Anti-Rabbit IgG (1:200, Proteintech) was added and incubated for 2 h at room temperature. Finally, the tissue specimens were stained with diaminobenzidine and exanimated with microscopic. Specimens were classified into negative, positive, ++ positive, or +++ positive, based on the sum of the staining intensity (varied from weak to strong) and staining extent scores.