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TALEN Beats CRISPR-Cas9 in Editing Tightly-packed DNA February 3, 2021
Genome editing tool known as TALEN was found to be 5 times more efficient than CRISPR-Cas9 at locating and editing genes in the densely-packed DNA called heterochromatin. Thus, it has more potential to address genetic defects in the heterochromatin such as sickle cell anemia,beta-thalassemia, fragile X syndrome, and others.
According to the article published in
Nature, the researchers from the University of Illinois at Urbana−Champaign used single-molecule imaging to observe the performance of genome-editing proteins in mammalian cells. This led them to the finding that Cas9 is less efficient in heterochromatin than TALEN because Cas9 becomes overloaded by local searches on non-specific sites in these regions. Furthermore, when TALEN was used instead of Cas9, the editing efficiency increased five times.