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Cell Migration and Invasion Assays by Platypus Technologies
In order to ensure consistent cell-free regions for each experiment, Cell Migration and Invasion Assays by Platypus Technologies utilize exclusion zone technology. The Oris™ Cell Migration and Invasion Assays allow the reproducible and accurate quantification of cell movement.
Cell Migration Assays
No artificial membranes
Industry-standard 96- or 384-well formats
Image Credit: Platypus Technologies, LLC
In order to form a central cell-free detection zone in the center of every well of a 96-well plate, Oris™ Cell Migration assays utilize physical “stopper” barriers.
Utilize Oris™ for maximal flexibility in assay design.
Utilize Oris™ with the Oris™ Detection Mask for analysis on plate readers that lack virtual masking.
Cell Migration Assays by Platypus Technologies
Cell Migration Assays from Platypus Technologies utilize exclusion-zone technology, ensuring that results are high quality and ideal for publication.
Researchers in both pharmaceutical companies and academia will benefit from the use of robust, powerful Cell Migration Assays by Platypus Technologies, particularly in studies looking to advance cancer research, drug discovery, or wound healing.
Cell Migration Assays from Platypus Technologies offer a range of benefits, including:
Industry-standard 96- or 384-well formats
Monitoring in real-time
Does not use artificial membranes
Oris™ allows for maximal assay flexibility
Oris™ Pro allows for complete automation with high Z factors
ECM Integrity Assay
In order to test the integrity of each assay’s ECM, 100 µg/mL of Collagen Type I – FITC conjugate (Sigma-Aldrich) was coated onto wells of an Oris™ assay plate (without stoppers) and a Costar
® plate. These plates were then incubated overnight at 37 °C / 5% CO
2.
After incubation, stoppers were inserted into the Oris™ assay plate while sterile PBS was added to both assay plates’ wells. At 1 hour, 24 hour, and 48 hour intervals post-insertion, stoppers were removed before fluorescence images were acquired using a Zeiss Axiovert inverted microscope.
Scratches were made in the Costar
® plate at each time point. Images were acquired using the same settings employed for the Oris™ assay plate.
An ultra-flat and pristine gold surface is provided by template-stripped gold chips for research applications, such as biosensor development, scanning electron microscope (SEM) and atomic force microscopy (AFM) and analyses of self-assembled monolayers.
The gold chips are produced by coating the flattest prime-grade silicon wafers available or a newly split mica, without an adhesion layer.
Following this, glass chips measuring 1 x 1 cm
2 are epoxy-glued to the gold surface. The gold surface employed by the users is the one that comes in contact with the mica or silicon substrate, and hence, it is ultra-flat and also protected from the air until it is ready to be used.