water is mixed with a nicotine leech eight and allowed to stand overnight. the research is then pulled this mixture into an aquarium. they introduce the young rainbow trout from the clean water aquarium into the nicotine both. after just a few seconds the fish reacts at fust it's just a few but then more and more are affected by the nicotine. they don't paralyze. some. of those tunnel mess side twitch. the creatures go into toxic shock.