Spatial biology techniques are advancing rapidly as powerful tools to gain an in-depth understanding of tissues and their cellular organization. In this application note, CrestOptics presents a high-speed four-camera set-up, based on CrestOptics confocal technology, used by Dr. Bayraktar’s group in the Wellcome Sanger Institute for spatial transcriptomics analysis. Dr. Bayraktar and collaborators developed and optimized a large-scale spatial transcriptomics pipeline, consisti