HPLC and UHPLC are both chromatographic techniques used to separate, identify and quantify components in liquid samples. Each component of the sample interacts differently with the adsorbent material in the chromatography columns being used, resulting in different flow rates for each component, causing them to be separated as they flow out of the column. HPLC and UHPLC have been used for pharmaceutical and biopharmaceutical manufacturing, forensic, research and medical purposes for ma