Spatially Resolved Transcriptomics Experiments Using a Spinning Disk Microscope Spatial transcriptomics (also known as multiplexing) is a cell biology technique able to uncover a number of (Xn) RNAs in their 2D or 3D biological context. Multiplexing is a popular approach in areas such as oncobiology, neurosciences, disease target diagnostics, development, and behavioral studies. A number of techniques have been developed to enable spatially resolved transcriptomics - also referred to as multiplexing or spatial genomics. These techniques include FISSEQ, osmFISH, instaSEQ, MERFISH, STARmap and seqFISH. Spatial transcriptomics offers a number of benefits, including its ability to provide spatial and sequence information for multiple gene products. Until recently, it was only possible to sequence RNAs in bulk, or at the single-cell level, with any tissue environment information (the biological context) lost during this process.