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"Soil environmental DNA metabarcoding in low-biomass regions requires p" by Pamela Olmedo-Rojas, Gert Jan Jeunen et al.

Environmental DNA is a powerful tool for monitoring biodiversity. Although environmental DNA surveys have successfully been implemented in various environments, protocol choice has been shown to affect results and inferences. Thus far, few method comparison studies for soil have been undertaken. Here, we optimized the workflow for soil metabarcoding through a comparative study encompassing variation in sampling strategy (individual and combined samples), DNA extraction (PowerSoil®, NucleoSpin® Soil, PowerSoil® + phosphate buffer and NucleoSpin® Soil + phosphate buffer) and library preparation (one-step and two-step quantitative polymerase chain reaction methods). Using a partial 18S rRNA marker, a total of 309 eukaryotic taxa across 21 phyla were identified from Antarctic soil from one site in the Larsemann Hills. Our optimized workflow was effective with no notable reduction in data quality for a considerable increase in time and cost efficiency. The NucleoSpin® Soil + phosphate buffer was the best-performing extraction method. Compared to similar studies in other regions, we obtained low taxonomic coverage, perhaps because of the paucity of Antarctic terrestrial organisms in genetic reference databases. Our findings provide useful methodological insights for maximizing efficiency in soil metabarcoding studies in Antarctica and other low-biomass environments.

Antarctica , 18s , Iodiversity , Iogeography , Esert , Dna , Perational-taxonomic-unit , Emote-environments ,

Match report: U18s put four past Everton in mini-derby victory

Liverpool U18s produced a magnificent display to claim a 4-0 victory over Everton in Saturday's mini-derby at the Kirkby Academy.

Oakley-cannonier , Melkamu-frauendorf , Martin-sherif , Stefan-bajcetic , Luca-stephenson , James-mcconnell , Cannonier-figueroa , Eddie-jones , Tommy-pilling , Zan-luk-leban , Bobby-clark , Clark-gyimah

Watch highlights: Weekend victories for U23s and U18s

Highlights of Liverpool U23s' 3-0 defeat of Arsenal U23s at Anfield are now available to watch on LFCTV GO.

James-norris , Aston-villa , Mateusz-musialowski , Paul-glatzel , League-cup , Premier-league , Academy , 18s , 23s ,

Blair nets the winner as LFC U18s beat Villa in Premier League Cup

Harvey Blair netted the winner as Liverpool U18s beat Aston Villa 2-1 away from home in the U18 Premier League Cup on Saturday afternoon.

Luke-chambers , Frauendorf-laffey , Bodymoor-heath , Stefan-bajcetic , Luca-stephenson , Crystal-palace , Marc-bridge-wilkinson , Academy , 18s ,

U18s match report: Liverpool 3-0 Sunderland

Liverpool U18s returned to winning ways in the U18 Premier League with a 3-0 defeat of Sunderland at the Academy.

Louie-salkeld , Oakley-cannonier , Melkamu-frauendorf , Luca-stephenson , Oliver-bainbridge , Harvey-davies , Harvey-blair , Tommy-pilling , Ben-middlemas , Bobby-clark , Luke-chamber , Academy

'Curtis Jones put on a show, it was great for the young lads to see'

Marc Bridge-Wilkinson has revealed how the club's Academy youngsters were inspired by Curtis Jones' display during Liverpool's 5-1 win over FC Porto this week.

Portugal , Porto , Melkamu-frauendorf , Tyler-morton , Curtis-jones , Barry-lewtas , Youth-league , Champions-league , Derby-county , Leeds-united , Academy , 18s

U18s beat Watford 5-0 in St. George's Park friendly

Oakley Cannonier netted twice as Liverpool U18s beat Watford 5-0 in a friendly encounter at St. George's Park on Saturday.

George-park , Harvey-blair , James-balagizi , Bobby-clark , Marc-bridge-wilkinson , Academy , 18s ,

LFC U18s put five past Stoke to kick off new season

Liverpool U18s came from a goal down to claim a thrilling 5-3 win over Stoke City in their opening match of the U18 Premier League season on Saturday.

Oakley-cannonier , Melkamu-frauendorf , Josh-davidson , Emre-tezgel , Luca-stephenson , James-balagizi , Harvey-davies , Mcconnell-blair , Matty-lusakueno , Stephenson-omoruyi , Lee-jonas ,

Explained: Jordan Henderson's surprise training session with LFC U18s

As captain of Liverpool FC, Jordan Henderson is a role model to thousands of youngsters worldwide.

Jordan , Aston-villa , Marc-bridge-wilkinson , Villa-park , Academy , Ordan-henderson , 18s , Arc-bridge-wilkinson , ஜோர்டான் , ஆஸ்டன்-வில்லா , மார்க்-பாலம்-வில்கின்சன்

Musialowski wins U18 Premier League Goal of the Season

Mateusz Musialowski has won the U18 Premier League Goal of the Season award.

League-football-development , Newcastle-united , Premier-league , Red-goal , Premier-league-football-development , Academy , 18s , Ateusz-musialowski , லீக்-கால்பந்து-வளர்ச்சி , நியூகேஸில்-ஒன்றுபட்டது , ப்ரிமியர்-லீக்