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In the race to create serum-free, chemically defined media, research efforts have focused on identifying the elements required for superior growth...
By Aziz Cayli, Burcu Tongul, Francis Nguyen, Adrien Lugari, and Érica Schulze BHK-21 cell strains have been utilized for several years as a platform for veterinary vaccines and recombinant protein manufacturing. However, these cells are anchorage-dependent, requiring the use of dissociation agents that slow and are traumatic for cells while also creating scale-up limitations. By contrast, suspension culture facilitates large-scale processes, offers options for process intensification and contributes to reduced manufacturing costs due to less process complexity. BHK-21 suspension cells grown ...