"Single-Molecule Fluorescence Methods to Study Protein Exchange Kinetic" by Richard R. Spinks, Lisanne M. Spenkelink et al.
Abstract Recent single-molecule studies have demonstrated that the composition of multi-protein complexes can strike a balance between stability and dynamics. Proteins can dynamically exchange in and out of the complex depending on their concentration in solution. These exchange dynamics are a key determinant of the molecular pathways available to multi-protein complexes. It is therefore important that we develop robust and reproducible assays to study protein exchange. Using DNA replication as an example, we describe three single-molecule fluorescence assays used to study protein exchange dy...
Source: uow.edu.au