"Methods and applications of single-molecule fluorescence microscopy" by Stefan H. Mueller
The molecular mechanisms that support life depend on the fate of small populations of molecules. The functions of biomolecules, however, are traditionally characterised through bulk-phase biochemical methods that only report on ensemble averages. Such methods are not sensitive to population heterogeneity or short-lived reaction intermediates. Single-molecule methods that allow for the direct observation of individual molecules have significantly advanced our understanding of biology in the past two decades. The goal of the work described in this PhD thesis was the development and use of single...