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D), ( E) Hydrodynamic diameters and Zeta potentials of cAuNPs and mAuNPs measured with dynamic light scattering (DLS). Note: hydrodynamic diameter and Zeta potential measurements are presented in the supporting information. Cellular Uptake of mAuNPs Shown in Figure 2A are photographs taken under dark field microscope of B16-F10 cells cultured with and without mAuNPs for 24 h. Numerous small spots in B16-F10 cells co-cultured with mAuNPs for 24 h were visible compared to controls, indicating that mAuNPs entered B16-F10 cells. Next, we investigated the cellular uptake of mAuNPs after B16-F10 cells were co-cultured with mAuNPs at different concentrations by ICP-AES (Figure 2B). The amount of cellular uptake of mAuNPs increased with the AuNPs co-culture concentrations in the range of ~0–60 μg/mL. The cellular uptake amount reached 3.6 pg/cell at the co-culture concentration of 10 μg/mL. If the weight of 10

United statesWu weiBax bclInstitute of modern physicsNational institutes of healthAdministration of aunpsChinese academy of sciences shanghaiBeckman coulter coCell bankThermo electron coJk chemical coImagej national institutes of healthIon therapy centerPrecision co ltdWuhan sanying biotechnology companySupporting information