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Polarized and wide-field light microscopy has been studied for many years to develop accurate and information-rich images within a focused framework on biophysics and biomedicine. Technological advances and conceptual understanding have recently led to significant results in terms of applications. Simultaneously, developments in label-free methods are opening a new window on molecular imaging at a low dose of illumination. The ability to encode and decode polarized light pixel by pixel, coupled with the computational strength provided by artificial intelligence, is the running perspective of l...

Many books on polarization give tables of Mueller matrices. The coherency matrix has been found useful for interpretetion of the Mueller matrix. Here we give a table of Mueller matrices M, coherency matrices C, and coherency matrix factors F for different polarization components and systems. F is not given for some complicated nondeterministic cases. In many cases, though, F has a very simple form. In particular, we give expressions for F for the general case of an homogeneous elliptic diattenuating retarder. Different coordinate systems for describing diattenuating retarders are compared, on ...

Depolarization has been found to be a useful contrast mechanism in biological and medical imaging. The Mueller matrix can be used to describe polarization effects of a depolarizing material. An historical review of relevant polarization algebra, measures of depolarization, and purity spaces is presented, and the connections with the eigenvalues of the coherency matrix are discussed. The advantages of a barycentric eigenvalue space are outlined. A new parameter, the diattenuationcorrected purity, is introduced. We propose the use of a combination of the eigenvalues of coherency matrices associa...

Abstract Many of the most important resolution improvements in optical microscopy techniques are based on the reduction of scattering effects. The main benefit of polarimetry-based imaging to this end is the discrimination between scattering phenomena originating from complex systems and the experimental noise. The determination of the coherency matrix elements from the experimental Mueller matrix can take advantage of scattering measurements to obtain additional information on the structural organization of a sample. We analyze the contrast mechanisms extracted from (a) the coherency matrix ...

In the field of physical optics, a propagating light beam is known as a propagating electromagnetic radiation, with the direction of propagation being horizontal to the direction of the electric and magnetic field vector. The polarizing direction of light is known as the direction of the electrical vector component. The light will be polarized in a direct manner if the route of the electrical field is consistent during this propagation. This demonstrates that the perpendicular components of this electric field vector are in phase, or there is no phase variation between them, as seen in Figure...