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Manitoba RCMP say the incident took place around 3:30 p.m. Thursday, when a car that was stopped at a traffic light at the Wenzel Road intersection was hit from behind by a semi.
According to police, the crash happened when a trailer became detached from the vehicle that was towing it during rush-hour traffic, causing a collision involving four other cars.
The closure affected Highway 101, between Henderson Highway and PTH 59.