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Immunexpress, Pty Ltd., a molecular diagnostic company focused on improving outcomes for suspected sepsis patients, today announced that SeptiCyte® RAPID has received regulatory clearance by the Australian Therapeutic Goods Administration (TGA). The clearance of the company's flagship medical device by the regulatory body allows for the sale, use and further commercial testing of SeptiCyte RAPID throughout Australia to differentiate infection-positive (sepsis) from infection-negative systemic in ....
Invasive infections due to group A Streptococcus (GAS) advance rapidly causing tissue degradation and unregulated inflammation. Neutrophils are the primary immune cells that respond to GAS infection, where it is known that the degree of the neutrophil inflammatory response can influence the outcome of infection. The response of human neutrophils to GAS has not been described in depth. This thesis aimed to characterise the neutrophil response to GAS using in vitro and in vivo models of invasive infection, to further understand the contribution of neutrophils to invasive GAS disease. The current project optimised methodology to investigate the presence of intracellular neutrophil proteins via immunoblot, including caspases, where increased proteases and phosphatase inhibitors decreased protein degradation in neutrophil lysates. Methods of immunoblot normalisation were assessed and revealed total protein is the most appropriate method when undertaking densitometric quantification of neutr ....
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