Ultrasensitive point-of-care immunoassay for secreted glycoprotein detects Ebola infection earlier than PCR
( B) Schematic of the GP gene transcription profile. ( C) Binding of scFv-phage clones to EBOV sGP measured using Ag-down ELISA. Each data point is N = 1 technical replicate for scFv-phage clones with a distinct sequence. a.u., arbitrary units. ( D) Binding of six scFv-Fc Abs to sGP, measured using Ag-down ELISA. Each data point represents the mean ± SEM of N = 2 replicates. ( E) Schematic of Ab pairing using the D4 assay. (i) cAb and FL-dAb printed onto POEGMA-coated glass are exposed to sGP-spiked sample. (ii) Fluorescent spots are imaged; insets show spots with high (white) and low (red...
Source: sciencemag.org