STED microscopy reveals sub-cellular details in living mice 06 Apr 2021 Yale project achieves super-resolution 3D imaging in native tissue environment. Spot the diference: 3D STED A group at Yale University has successfully captured 3D super-resolution images from the brains of living mice. As published in Optica, the breakthrough was based on the use of stimulated emission depletion (STED) microscopy, one of the family of techniques developed to allow imaging beyond the normal diffraction limit of optical microscopy. The principles behind STED, for which Stefan Hell shared the 2014 Nobel p...