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"Rapid and High Throughput Hydroponics Phenotyping Method for Evaluatin" by Muhammad A. Asif, Sean L. Bithell et al.

Phytophthora root rot (PRR) is a major constraint to chickpea production in Australia. Management options for controlling the disease are limited to crop rotation and avoiding high risk paddocks for planting. Current Australian cultivars have partial PRR resistance, and new sources of resistance are needed to breed cultivars with improved resistance. Field- and glasshouse-based PRR resistance phenotyping methods are labour intensive, time consuming, and provide seasonally variable results; hence, these methods limit breeding programs’ abilities to screen large numbers of genotypes. In this study, we developed a new space saving (400 plants/m2), rapid (<12 >days), and simplified hydroponics-based PRR phenotyping method, which eliminated seedling transplant requirements following germination and preparation of zoospore inoculum. The method also provided post-phenotyping propagation all the way through to seed production for selected high-resistance lines. A test of 11 diverse chi ....

High Throughput , Ydroponics Screening Method , Phytophthora Root Rot , Plant Breeding , Uantitative Resistance Phenotyping , Oot Disease Resistance Breeding ,