Kingdom FM
It aims to identify positive Covid-19 cases and break chains of transmission.
Anyone with no symptoms of the virus, but who might be infectious, will be offered testing.
There will be a focus on communities where there is sustained transmission or a need for testing.
Pop-up testing sites will start to be rolled out in early February, with additional mobile testing also undertaken to respond to outbreaks.
Chief Executive of NHS Fife Carol Potter said: "This is an important expansion of testing which aims to help reduce Covid-19 rates in Fife, by identifying those who are positive cases but show no symptoms.