18) and quality trimmed by using Trimmomatic version 0.36 (
19). Viral contigs were generated by using default settings with Vicuna version 1.1 (
20), and a de novo consensus assembly was generated by using Viral Finishing and Annotation Toolkit (V-FAT) version 1.1 (https://www.broadinstitute.org/viral-genomics/v-fat). Read data are available from the National Center for Biotechnology Information Read Archive (https://www.ncbi.nlm.nih.gov) under BioProject accession no. PRJNA661611.
Phylogenetic Reconstruction
21) and IQ-TREE version 1.6 (
22). We then constructed a maximum-likelihood phylogenetic tree with 1,000 ultrafast bootstrap replicates (
HCV Transmission Network Analyses
We uploaded Illumina paired-end reads to GHOST and subjected them to automatic quality control criteria. In brief, read pairs were filtered out if a read had 3 Ns (N indicates that software was not able to make a basecall for this base) or a length 185 bp. Each identifier on forward and reverse reads
2020 vision: Research communicators pick their most memorable stories December 17, 2020
It remains without saying that 2020 has offered unprecedented challenges. In the earliest weeks of the COVID-19 pandemic, classrooms emptied, and instruction and outreach quickly transitioned to remote delivery. Research labs, ultimately reopened with modifications for health and safety, were closed for nearly all but those researchers studying the SARS-CoV-2 virus and its effects.
Yet, our community is filled with examples of resilience, dedication and passion. We have the privilege of covering the work of its researchers and students, whose efforts seem more important than ever as we confront the realities of a pandemic. This year has reminded all of us just how much science, and its connection to humanity, influences our lives. Here are the stories that stood out most to us this year.
Health department launches new data dashboard hngnews.com - get the latest breaking news, showbiz & celebrity photos, sport news & rumours, viral videos and top stories from hngnews.com Daily Mail and Mail on Sunday newspapers.
Sewage Now Being Used To Predict Coronavirus Cases - Milwaukee, WI - For people with COVID-19, the virus can be detected in their feces shortly after they are infected with the virus, the DHS explained.