Page 36 - Application Note News Today

Reversed-phase liquid chromatography (RPLC) is often associated with quantitative or analytical-scale analysis, but it is also a useful preparative scale tool for purification. For any purification project, there are three possible objectives: purity, yield, and throughput. These objectives are related and are commonly represented as a triangle. Two of these targets can be achieved at the expense of the third. Prioritizing the objectives of the purification project before developing the met

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Plasma is a rich source for protein biomarkers that can be used to reveal disease biology, measure responses to therapeutic treatments, or for diagnostic and prognostic purposes. A major advantage of plasma proteomics is the convenience of obtaining samples from routine blood draws and the availability of large cohorts of samples stored awaiting analysis such as in biobanks. However, identifying these biomarkers with bottom-up proteomics has substantial c

In this application note, PHC Corporation of North America presents its VIP ® ECO SMART series of ultra-low temperature freezers, featuring advanced variable speed inverter compressor technology and integrated electronics, reducing energy consumption by nearly 30% over previous models. The series offers enhanced security features, such as electric lock function and multiple authentication systems, maki

In recent years there has been a shift to analyzing smaller sample quantities, driven by the interest in profiling the proteome of individual cells. This increased focus on LC-MS analysis of limited sample amounts (e.g., single-cells) requires the highest possible sensitivity. At the same time, throughput and chromatographic performance must be preserved to generate sufficiently high-quality data from large data sets to draw meaningful conclusions. Traditional proteomics approaches read out the

In this application note, Illumina highlights the upcoming advancements in genomics. Over the next 25 years, it predicts breakthroughs in cardiovascular disease testing, AI-driven drug target discovery, and expanded testing capabilities for tuberculosis-affected countries. Its customers are also making strides in research for autism spectrum disorder, PTSD, and diabetes. In the note, Illumina also celebrates its quarter-century involvement in the global genomics movement and looks forward to fur

In this application note, Waters Corporation addresses the limitations of stainless steel HPLC columns due to corrosion and their negative impact on peak shape and analyte recovery. While alternative materials like titanium and nickel-cobalt alloys offer improved corrosion resistance, they still pose challenges for certain analyses. The interaction between analytes and metal surfaces can lead to poor peak shape, analyte losses, and inaccuracies.  To overcome these issues, chromatographers o

PFAS are per- and polyfluoroalkylated substances with persistent properties, which have therefore been and still are used in everyday objects (e.g., water-repellent coatings for textiles and paper, cookware). Due to their multiple uses, PFAS are currently found in numerous environmental samples. Besides monitoring of surface water or contaminated soils, control at wastewater treatment plants is of increasing interest. Since PFAS are very resistant to conventional biological treatment in wastewat

The need for high-throughput sample acquisition and automation in flow cytometry creates demand for new methods for analytical standardization and for reducing sources of variability. When performed on the Attune CytPix Flow Cytometer, automated image analysis combined with conventional flow cytometry provides a robust quantitative method for standardizing flow cytometry data analysis. In this application note, Thermo Fisher Scientific demonstrates that automated image-assisted analysis enabl

Cephem antibiotics like penicillin and carbapenems are a type of β-lactam antibiotic used in oral medicines and injectable solutions. Cephem antibiotics are said to be effective for various illnesses because they have a high safety profile with a low frequency and degree of side effects. In this application note, Shimadzu introduce a simultaneous analysis method for cephem antibiotics in blood plasma by LC-MS/MS using the CLAM-2030, which enables fully-automated pretreatment of biologic