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Scientists discover how an enzyme involved in DNA repair becomes vital to tumor survival

Scientists at the Francis Crick Institute, in collaboration with Artios, have identified how an enzyme involved in DNA repair (POLQ), becomes vital to the survival of certain cancers, if the cancer cells lose the ability to use a more common method of DNA repair.
United Kingdom Simon Boulton Ondrej Belan Emily Henderson Lab Diagnostics Automation Lab Diagnostics Automation Industry

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"Methods and applications of single-molecule fluorescence microscopy" by Stefan H. Mueller

The molecular mechanisms that support life depend on the fate of small populations of molecules. The functions of biomolecules, however, are traditionally characterised through bulk-phase biochemical methods that only report on ensemble averages. Such methods are not sensitive to population heterogeneity or short-lived reaction intermediates. Single-molecule methods that allow for the direct observation of individual molecules have significantly advanced our understanding of biology in the past ...
Single Molecule Dna Replication Fluorescence Microscopy
Source: uow.edu.au

Common molecular tool for DNA labelling shows unexpected anticancer properties

Scientists at the UNC School of Medicine have made the surprising discovery that a molecule called EdU, which is commonly used in laboratory experiments to label DNA, is in fact recognized by human cells as DNA damage, triggering a runaway process of DNA repair that is eventually fatal to affected cells, including cancer cells.
Sarah Graham Kenan Aziz Sancar Emily Henderson School Of Medicine Lineberger Comprehensive Cancer Center Proceedings Of The National Academy Sciences

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