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"Preparation of the Murine Anti-Human P2X7 Receptor Monoclonal Antibody" by Amal Elhage, Ross J. Turner et al.

The murine anti-human P2X7 receptor monoclonal antibody (mAb) (clone L4) has been used to study the expression and function of the P2X7 receptor on primary leukocytes, keratinocytes, osteoblasts and neuronal cells, as well as various cell lines. This antibody has also been used to characterize polymorphic variants and isoforms of the P2RX7 gene and P2X7 site-directed mutations, and to identify molecules coassociated with P2X7 in the plasma membrane. This chapter describes the maintenance and cry...
Antibody Conjugation Antibody Purification B Lymphocytes Flow Cytometry Monoclonal Antibody 2x7 Receptor
Source: uow.edu.au

"Humanized Mouse Model to Study the P2X7 Receptor in Graft-Versus-Host " by Debbie Watson, Sam R. Adhikary et al.

Humanized mouse models of graft-versus-host disease (GVHD), where human immune cells are injected into immune deficient mice, are well established and provide opportunities to investigate pathways involved in GVHD development. This chapter provides an overview of human immune cell isolation, injection of these cells into immune deficient mice, monitoring of mice for signs of GVHD, and assessment of human cell engraftment using flow cytometry. Further, this chapter focuses on the P2X7 signaling p...
Flow Cytometry Hematopoietic Stem Cell Transplantation Human Peripheral Blood Mononuclear Cells Sg Mice Enogeneic Graft Versus Host Disease
Source: uow.edu.au

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