"Humanized Mouse Model to Study the P2X7 Receptor in Graft-Versus-Host " by Debbie Watson, Sam R. Adhikary et al.
Humanized mouse models of graft-versus-host disease (GVHD), where human immune cells are injected into immune deficient mice, are well established and provide opportunities to investigate pathways involved in GVHD development. This chapter provides an overview of human immune cell isolation, injection of these cells into immune deficient mice, monitoring of mice for signs of GVHD, and assessment of human cell engraftment using flow cytometry. Further, this chapter focuses on the P2X7 signaling p...
Flow Cytometry Hematopoietic Stem Cell Transplantation Human Peripheral Blood Mononuclear Cells Sg Mice Enogeneic Graft Versus Host Disease
Source: uow.edu.au