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"Methods and applications of single-molecule fluorescence microscopy" by Stefan H. Mueller

The molecular mechanisms that support life depend on the fate of small populations of molecules. The functions of biomolecules, however, are traditionally characterised through bulk-phase biochemical methods that only report on ensemble averages. Such methods are not sensitive to population heterogeneity or short-lived reaction intermediates. Single-molecule methods that allow for the direct observation of individual molecules have significantly advanced our understanding of biology in the past ...
Single Molecule Dna Replication Fluorescence Microscopy
Source: uow.edu.au

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Novel algorithm provides rich, detailed information on protein location and function within a cell

Humans are good at looking at images and finding patterns or making comparisons. Look at a collection of dog photos, for example, and you can sort them by color, by ear size, by face shape, and so on. But could you compare them quantitatively? And perhaps more intriguingly, could a machine extract meaningful information from images that humans can't?
Chan Zuckerberg Biohub Hirofumi Kobayashi Manuel Leonetti Loic Royer Biohub Opencell Emily Henderson

CrestOptics' DeepSIM super resolution confocal microscope module named by microscopy today as a top 10 best microscopy innovation of 2022

CrestOptics S.p.A., a manufacturer of high-end microscopy solutions and advanced systems for fluorescence microscopy and diagnostic applications, has been recognised as developing one of the ten best microscopy innovations in the 2022 Microscopy Today Innovation Awards, DeepSIM.
United States Raino Ceccarelli Microscopy Society Of America Head Of Product Development Today Innovation Awards Microscopy Today

Scientists discovere how a protein and enzymes interact to allow hepatitis A virus replication

The viral replication cycle is crucial for a virus to spread inside the body and cause disease. Focusing on that cycle in the hepatitis A virus (HAV), UNC School of Medicine scientists discovered that replication requires specific interactions between the human protein ZCCHC14 and a group of enzymes called TENT4 poly(A) polymerases.
United States San Diego Emily Henderson School Of Medicine Walter Reed Army Medical Center Proceedings Of The National Academy Sciences

Shapeshifting behavior of E. coli may offer clues to combat urinary tract infections

Urinary tract infections are both very common and potentially very dangerous. More than half of all Australian women will suffer from a UTI in their lifetime, and nearly one in three women will have an infection requiring treatment with antibiotics before the age of 24.
New South Wales Iain Duggin Emily Henderson Nature Communications Australian Institute For Microbiology University Of Technology Sydney

Bruker introduces enhanced 3D holographic stimulation for optogenetics on its Ultima 2Pplus multiphoton microscope

Bruker Corporation today announced the release of the NeuraLight 3D Ultraโ„ข module to support advanced neuroscience and optogenetics research applications on Brukerโ€™s Ultima multiphoton microscopes.
Bruker Nano Surfacesjun Adam Packer Xiaomei Li University Of Oxford Wellcome Trust Sir Henry Dale Bruker Corporation

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