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CRISPR Screen and Deep Sequencing Map DNA Repair Landscape Boosting Genome Editing Prospects

Although genome editing holds unprecedented promise in the treatment of diseases, one of the major obstacles is that existing technologies frequently result in unwanted mutations or can fail to introduce any changes at all. Now, a new resource called Repair-seq has been developed, and it allows scientists to investigate how the complex cellular DNA repair mechanisms influence the outcome of genome editing technologies. Repair-seq provides a starting point for further explorations of DNA repair p...
Jeffrey Hussmann John Doench Britt Adamson Jonathan Weissman Broad Institute Genetic Perturbation Program

Princeton-led studies boost CRISPR gene-editing prospects

A Princeton-led team of researchers from the University, MIT, Harvard, and Editas Medicine developed and applied a new method to understand the activity of cellular genes involved in correcting DNA damage, paving the way for dramatic improvements to genome-editing technologies. These advances are described in a pair of papers that appeared online Oct. 14 and Oct. 20, 2021, in the journal "Cell."
David Liu Caitlin Sedwick Sameera Khan Fotobuddy John Doench Britt Adamson Jonathan Weissman

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