The Centers for Disease Control and Prevention contracted with laboratories to sequence the SARS-CoV-2 genome from positive samples across the United States to enable public health officials to investigate the impact of variants on disease severity as well as the effectiveness of vaccines and treatment. Herein we present the initial results correlating RT-PCR quality control metrics with sample collection and sequencing methods from full SARS-CoV-2 viral genomic sequencing of 24,441 positive patient samples between April and June 2021. RT-PCR confirmed (N Gene Ct value < 30) positive patient samples, with nucleic acid extracted from saliva, nasopharyngeal and oropharyngeal swabs were selected for viral whole genome SARS-CoV-2 sequencing. Sequencing was performed using Illumina COVIDSeq™ protocol on either the NextSeq550 or NovaSeq6000 systems. Informatic variant calling, and lineage analysis were performed using DRAGEN COVID Lineage applications on IlluminaR
Frontiers | The First Molecular Characterization of Serbian SARS-CoV-2 Isolates From a Unique Early Second Wave in Europe frontiersin.org - get the latest breaking news, showbiz & celebrity photos, sport news & rumours, viral videos and top stories from frontiersin.org Daily Mail and Mail on Sunday newspapers.
Plumbing the ‘dark’ genome for new genes
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This can aid diagnosis, cure diseases
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New revelaton: Mutations in the novel regions do have physiological consequences, says Sudhakaran Prabakaran.
| Photo Credit:
Rasi Bhadramani
This can aid diagnosis, cure diseases
On June 26, 2000, former U.S. President, Bill Clinton, announced the completion of a draft sequence of the human genome, a historic landmark for genetic research. The Human Genome Project helped map our genes, strengthened the study of human diseases and aided new drug discovery. But even after two decades, the number of ‘known’ genes – encoding around 20,000 ‘known’ proteins - has remained constant. It is also a conundrum why only 1.5% of the entire human genome codes for proteins.
by Aravindan Neelakandan - Feb 12, 2021 10:57 AM
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The work that Sudhakaran Prabakaran and his team have done over the past five years strongly suggests that our definition of a gene has been extremely conservative.
If physicists have their dark matter, molecular biologists have their dark DNA or dark genome.
Now a dynamic team of young biologists from across the planet, the Prabakaran Group, is cracking its secrets like never before.
Let s start from the basics.
What is a gene?
The gene is that part of the DNA strand that codes for a protein.
That is a strict and conservative textbook definition. But then, for quite a considerable number of years now, scientists studying the genome have been facing a problem.