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Pooled CRISPR screening identifies m6A as a positive regulator of macrophage activation

Cd86), were down-regulated in Mettl3-deficient cells (Fig. 1G), suggesting that METTL3 has a critical function in controlling the innate immune response of Raw 264.7 macrophages. To further confirm the biological role of the m 6A modification in macrophages, Mettl3 conditional KO (CKO) mice were generated by crossing Mettl3 flox/flox mice with mice expressing Cre recombinase under the control of lysozyme 2 promoter ( Lyzm-Cre). We have documented the loss of both the METTL3 protein and the o...
United States Agilent Bioanalyzer Amersham Hybond Merck Millipore Hua Bing Li Lipofectamine Rnaimax Invitrogen

Frontiers | Quantification of Huntington's Disease Related Markers in the R6/2 Mouse Model

2Department of Health Studies, FH Joanneum University of Applied Sciences, Graz, Austria Huntington’s disease (HD) is caused by an expansion of CAG triplets in the huntingtin gene, leading to severe neuropathological changes that result in a devasting and lethal phenotype. Neurodegeneration in HD begins in the striatum and spreads to other brain regions such as cortex and hippocampus, causing motor and cognitive dysfunctions. To understand the signaling pathways involved in HD, animal models ...
United States Panreac Applichem Gmb Zeiss Axiocam Graphpad Software Inc Vector Laboratories Inc Accreditation Of Laboratory Animal Care

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