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Reversed-phase liquid chromatography (RPLC) is often associated with quantitative or analytical-scale analysis, but it is also a useful preparative-scale tool for purification. For any purification project, there are three possible objectives: purity, yield, and throughput. These objectives are related and are commonly represented as a triangle. Two of these targets can be achieved at the expense of the third. Prioritizing the objectives of the purification project before developing the met