A 2-year, large-scale trial in beef cattle in Alberta, Canada has successfully demonstrated that a novel feed ingredient, developed by Royal DSM, can be included in commercial feedlot diets to reduce methane emissions by up to 80%, without negative effects on animal health and performance parameters and carcass characteristics. This was the largest and longest trial for methane reduction in beef to date. The trial alone already reduced Greenhouse Gas (GHG) emissions by 1,473 metric tons carbon dioxide equivalents (CO2e). This is comparable to taking 500 cars off the road for a year. The trial was conducted by a Canadian Research Consortium consisting of Agriculture and Agrifood Canada, Feedlot Health Management Services, Viresco Solutions, and DSM Nutritional Products, and with support from the Alberta Cattle Feeders Association. Emissions Reduction Alberta (ERA) committed $1.5 million to the $3 million project through its Methane Challenge. The project was recognized for having positive implications for the province due to the fact 70% of Canada’s cattle production happens in Alberta. With approximately 15,000 head of beef cattle included in the trial, it represents the largest single trial conducted on methane reduction technologies for ruminants.