Hsa_circRNA_000543 predicts poor prognosis - CMAR
Luciferase Reporter Assays The circ-000543 wild sequence, ZNF268-3′UTR wild sequence and their mutations with or without miR-567 binding sites were synthesized and then subcloned into luciferase reporter vectors psiCHECK-2, which was bought from Promega (Madison, WI, USA). HEK293T cells were implanted in 24-well plates at the density of 3×10 4 cells/well in triplicate. Then, miR-567 or NC mimic were cotransfected with corresponding psiCHECK-2 plasmids. After co-transfection for 48 h, dual-luciferase reporter assay system that provided by Promega was proceeded to conduct the luciferase repo...
Source: dovepress.com