Using CellDrop to Count Isolated Nuclei
Using CellDrop to Count Isolated Nuclei Sponsored Content by DeNovix Inc.Mar 5 2021 The successful isolation of nuclei is an essential step in both ATAC-sequencing workflows and single cell RNA-sequencing. Successful library preparation requires users to ensure that all sample input is non-clustered and debris-free. Dual fluorescence counting using the CellDrop™ FL Automated Cell Counter is a useful tool, able to distinguish unlysed intact cells from isolated nuclei. Cluster analysis software provides an aggregation estimate to the user, with the resulting images allowing the user to verify...
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