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Using CellDrop to Count Isolated Nuclei Sponsored Content by DeNovix Inc.Mar 5 2021 The successful isolation of nuclei is an essential step in both ATAC-sequencing workflows and single cell RNA-sequencing. Successful library preparation requires users to ensure that all sample input is non-clustered and debris-free. Dual fluorescence counting using the CellDrop™ FL Automated Cell Counter is a useful tool, able to distinguish unlysed intact cells from isolated nuclei. Cluster analysis software provides an aggregation estimate to the user, with the resulting images allowing the user to verify...

Quantifying Isolated Nuclei from Mouse Brain Using the CellDrop™ FL Automated Cell Counter Isolating nuclei is an essential step in both single nuclear RNA-sequencing and ATAC-sequencing workflows. It is important to ensure that samples obtained during library preparation are non-clustered and debris-free, but nuclei isolated from tissue samples in areas like the brain are prone to high debris in comparison with samples obtained from cultured cells. It is also important that nuclei are accurately counted for single cell analysis, even in instances where debris does not impact upon library p...