Quantifying Isolated Nuclei from Mouse Brain Using the CellDrop™ FL Automated Cell Counter
Quantifying Isolated Nuclei from Mouse Brain Using the CellDrop™ FL Automated Cell Counter Isolating nuclei is an essential step in both single nuclear RNA-sequencing and ATAC-sequencing workflows. It is important to ensure that samples obtained during library preparation are non-clustered and debris-free, but nuclei isolated from tissue samples in areas like the brain are prone to high debris in comparison with samples obtained from cultured cells. It is also important that nuclei are accurately counted for single cell analysis, even in instances where debris does not impact upon library p...
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